In contrast to random primers, oligo (dT) primers are usually not well suited for degraded samples but are great for setting up cDNA libraries and suggested to be used with reverse transcriptases (RTs) as Superscript IV RT.Enable’s begin, but very first…just a little disclaimer this is solely educational. Choose treatment in reloading since it
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I’ve only used “hard” primers for instance Tula and Sellier & Bellot just because I discovered a killer offer on them (from Cabela’s).Swimming pools of pre-created primers may also help generate extensive expression profiles, Specially wherever assay sensitivity is critically significant.Stay away from storing primers close to chemical comp
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